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KMID : 0380620090410010093
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Korean Journal of Food Science and Technology
2009 Volume.41 No. 1 p.93 ~ p.99
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Protective Effect of Glycoprotein Isolated from Cudrania tricuspidata on Liver in CCl4-treated A/J Mice
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Joo Heon-Yeong
Lim Kye-Taek
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Abstract
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This study aimed to determine whether or not glycoprotein isolated from Cudrania tricuspidata Bureau fruit(CTBglycoprotein) exerts a hepatoprotective effect on liver injury induced by the administration of carbon tetrachloride(CCl4,1.0mL/kg) to A/J mice. Following the administration of CTB glycoprotein(0-20mg/kg), the activities of antioxidant enzymes(superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GPx)), and the quantities of measured thiobarbituricacid reactive substances(TBARS), lactate dehydrogenase(LDH), and nitric oxide(NO) were evaluated from the murine livertissues and plasma. Additionally, the activity of nuclear factor-kappa B(NF-κB) was assessed after pretreatment with CCl4.When the mice were treated with CCl4 alone, the activities of antioxidative enzymes reduced but amounts of TBARS,LDH, and NO increased. However, the results of treatment with CTB glycoprotein(10 and 20mg/kg) revealed significantlyincreased activities of antioxidant enzymes(SOD, CAT, and GPx), as compared with CCl4 alone. On the other hand, theresult showed significant diminutions of the quantities of TBARS, LDH, and NO after treatment with CTB glycoprotein(10and 20mg/kg), as compared to CCl4 alone. The activity of NF-κB also declined after pretreatment with CTB glycoprotein, ascompared with CCl4 treatment alone. Thus, it is suggested that the CTB glycoprotein exerts a protective effect against CCl4-induced liver injury in A/J mice.
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KEYWORD
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Cudrania tricuspidata Bureau(CTB) glycoprotein, antioxidant enzyme, lactate dehydrogenase, nitric oxide, nuclearfactor-kappa B
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